from 01.01.2023 until now
from 01.01.2025 until now
employee
VAK Russia 4.1.1
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
VAK Russia 4.3.3
VAK Russia 4.3.5
UDC 634.71
The objective of the research is to improve the technology for adapting and further growing planting material of European cranberry (Vaccinium oxycoccos L.) to ex vitro conditions. The work was conducted at the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy in 2024-2025. The objects of the study are V. oxycoccos plants of the Krasa Severa and Fomich cultivars obtained by clonal micropropagation. Rooted in vitro microclones of V. oxycoccos were planted in mini-greenhouses for adaptation. Substrate selection and preparation, plant preparation, and cultivation were carried out according to generally accepted methods. Rooting substrates used included high-moor peat (pH = 2.8–3.1), as well as mixtures of peat + river sand (3 : 1), peat + vermiculite (3 : 1), peat + agroperlite (3 : 1), and peat + natural volcanic zeolite (3 : 1). V. oxycoccos plants were transplanted into 1- and 2-liter containers in the first ten days of June and further grown indoors. Biometric measurements were taken at the end of each ten-day period for three months, and their survival rate to non-sterile ex vitro conditions was determined. The best adaptation of regenerated V. oxycoccos plants to non-sterile conditions by the 30th day was achieved on a substrate of a 3 : 1 mixture of peat and zeolite, and a 3 : 1 mixture of peat and agroperlite. The survival rate of V. oxycoccos was 97–99 %. The Fomich cultivar proved somewhat more resilient to the stress of transplanting plants into larger pots. Container size was found to have no significant effect on the quality of the planting material of the Russian V. oxycoccos cultivars studied.
Vaccinium oxycoccos, cranberry, adaptation, ex vitro, biometric indicators, survival rate, container growing
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2. Makarov SS, Kuznetsova IB, Upadyshev MT, et al. Clonal micropropagation of cranberry (Oxycoccus palustris Pers.). Food Production Engineering and Technology. 2021;51(1):67-76. (In Russ.). DOI:https://doi.org/10.21603/2074-9414-2021-1-67-76.
3. Nechiporenko IV, Akimova SV, Kazakov PO. Peculiarities of ex vitro growing completion of bog cranberry (Vaccinium oxycoccos L.) with a closed root system. Vegetable Crops of Russia. 2023;5:49-61. (In Russ.). DOI:https://doi.org/10.18619/2072-9146-2023-5-49-61.
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8. Anjanappa RB, Gruissem W. Current progress and challenges in crop genetic transformation. J Plant Physiol. 2021;261:153411. DOI:https://doi.org/10.1016/j.jplph.2021.153411.
9. Dhungana SA, Kunitake H, Niino T, et al. Cryopreservation of blueberry shoot tips derived from in vitro and current shoots using d cryo-plate technique. Plant Biotechnol (Tokyo). 2017;34(1):1-5. DOI:https://doi.org/10.5511/plantbiotechnology.16.1231b.
10. Marangelli F, Pavese V, Vaia G, et al. In vitro polyploid induction of highbush blueberry through de novo shoot organogenesis. Plants (Basel). 2022;11(8):2349. DOI:https://doi.org/10.3390/plants11182349.
11. Makarov SS, Cheryatova YuS, Chudetsky AI. Experience of Introducing large-fruited cranberry (Vaccinium macrocarpon Ait.) when growing in the Moscow Region. Bulletin of KSAU. 2025;4:33-46. (In Russ.). DOI:https://doi.org/10.36718/1819-4036-2025-4-33-46.
12. Liaudanskas M, Šedbarė R, Janulis V. Determination of biologically active compounds and antioxidant capacity in vitro in fruit of small cranberries (Vaccinium oxycoccos L.) growing in natural habitats in lithuania. Antioxidants (Basel). 2024;13(9):1045. DOI:https://doi.org/10.3390/antiox13091045.
13. Šedbarė R, Janulis V, Pavilonis A, et al. Comparative in vitro study: assessing phytochemical, antioxidant, antimicrobial, and anticancer properties of Vaccinium macrocarpon Aiton and Vaccinium oxycoccos L. fruit extracts. Pharmaceutics. 2024;16(6):735. DOI:https://doi.org/10.3390/pharmaceutics16060735.
14. Pavlova IA, Gavrilenko IV, Matyash YuS, et al. Factors of effective adaptation of grape in vitro plants to ex vitro conditions. Magarach. Viticulture and Winemaking. 2021;23(3):226-232. (In Russ.). DOI:https://doi.org/10.35547/IM.2021.30.22.003.
15. Sungurova NR, Makarov SS, Chudetsky AI, et al. Adaptation features of kamchatka bilberry (Vaccinium praestans Lamb.) plants grown in vitro. Russian Forestry Journal. 2025;3:121-131. DOI:https://doi.org/10.37482/0536-1036-2025-3-121-131.
16. Perchenko NA, Sergeeva ON. Selection of optimal techniques for successful adaptation and further growing of plants in vivo for mass production of honeysuckle planting material with a closed root system. Agrarnaya Rossiya. 2024;1:33-35. (In Russ.). DOI:https://doi.org/10.30906/1999-5636-2024-1-33-35.
17. Méndez-Hernández HA, Loyola-Vargas VM. Plant micropropagation and temporary immersion systems. Methods Mol Biol. 2024;2827:35-50. DOI:https://doi.org/10.1007/978-1-0716-3954-2_3.
18. Sundyreva MA, Rebrov AN, Mishko AE, et al. Influence of sucrose concentration in the culture medium and weak stresses on the photosynthetic parameters of in vitro cultured grape plantlets during ex vitro acclimatization. Fruit Growing and Viticulture of South Russia. 2023;79:108-127. DOI:https://doi.org/10.30679/2219-5335-2023-1-79-108-127. (In Russ.).
19. Makarov SS, Chudetsky AI, Cheryatova YuS, et al. Features of adaptation of micro plants Rubus arctucus L. to ex vitro and in vivo conditions. Dostizheniya nauki i tekhniki APK. 2025;39(3):45-50. (In Russ.). DOI:https://doi.org/10.53859/02352451_2025_39_3_45.
20. Makarov SS, Samoylenko ZA, Makarova TA, et al. Adaptation of american cranberry (Vaccinium macrocarpon Ait.) to ex vitro conditions using the hydroponic method. Bulletin of KSAU. 2023;11:104-112. (In Russ.). DOI:https://doi.org/10.36718/1819-4036-2023-11-104-112.
21. Mazurek M, Siekierzyńska A, Piechowiak T, et al. Comprehensive analysis of highbush blueberry plants propagated in vitro and conventionally. Int J Mol Sci. 2023;25(1):544. DOI:https://doi.org/10.3390/ijms 25010544.
22. Schuchovski C, Sant'Anna-Santos BF, Marra RC, et al. Morphological and anatomical insights into de novo shoot organogenesis of in vitro 'Delite' Rabbiteye blueberries. Heliyon. 2020;6(11):e05468. DOI:https://doi.org/10.1016/j.heliyon.2020.e05468.
