from 01.01.2021 until now
Tver', Tver, Russian Federation
from 01.01.2020 until now
Tver', Tver, Russian Federation
from 01.01.2020 until now
Tver', Tver, Russian Federation
Tver, Tver, Russian Federation
employee from 01.01.2004 until now
Tver, Tver, Russian Federation
VAK Russia 4.3.3
VAK Russia 4.3.5
VAK Russia 4.1.1
VAK Russia 4.1.2
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
UDC 633.85
UDC 665.3
UDC 664.34
UDC 543.421
UDC 543.424
The aim of the study is to comparatively evaluate the properties of vegetable oils obtained by pressing and chemical extraction methods. Objectives: conducting an organoleptic evaluation of pressed commercial oils and laboratory extraction samples; determining standard quality indicators; analyzing the content of carotenoids and chlorophylls using UV spectroscopy. Research was conducted at the Bast Crops Processing Laboratory of the Federal Scientific Center for Bast Crops in Tver. The object of the study was pressed and extracted oils of flax, hemp, rapeseed, and black cumin. The study found that the extraction method provides a higher oil yield (up to 45 %) compared to pressing (up to 25 %). However, achieving a higher yield is associated with changes in product characteristics. According to the results of the organoleptic evaluation, the extracted oils have a rich color, but are inferior to pressed samples in the following respects: they are characterized by a less pronounced odor with the presence of traces of solvent and less transparency. The acid number of the extracted oils naturally exceeded the value of this indicator in pressed oils for all the studied crops, while the values of the iodine number and moisture content of all samples corresponded to the established standards. UV spectroscopy confirmed the presence of a pigment complex in the oils studied. All samples exhibited absorption peaks in the 400–500 nm range, characteristic of carotenoids. An additional peak in the 670 nm range was detected in the extracted oils, indicating the presence of chlorophylls, which correlates with their more intense color. These results confirm that the choice of extraction method is a determining factor influencing the yield, quality, and consumer properties of vegetable oils. The identified patterns can be used to optimize oilseed processing technology depending on the intended use of the final product.
oilseed crops, vegetable oil, carotenoids, chlorophyll, spectrophotometry
1. Agroe`ksport. Semena l`na. Obzor VE`D. 03.07.2024. Available at: https://aemcx.ru/wp-content/uploads/2024/07/obzor_ved_semena-lna.pdf Accessed: 18.08.2025. (In Russ).
2. Ploshhadi maslichny`x kul`tur v 2025 godu rekordno vy`rosli. Available at: https://ab-centre.ru/news/ploschadi-maslichnyh-kultur-v-2025-godu-rekordno-vyrosli. Accessed: 20.08.2025. (In Russ).
3. The SS, Birch J. Physicochemical and quality characteristics of cold-pressed hemp, flax and canola seed oils. Journal of Food Composition and Analysis. 2013;30:26-31. DOI:https://doi.org/10.1016/j.jfca.2013.01.004.
4. Kara SM, Gul V, Kiralan M. Fatty acid composition of hempseed oils from different locations in Turkey. Spanish Journal of Agricultural Research. 2010;8(2):385-390. DOI:https://doi.org/10.5424/sjar/2010082-1220.
5. Safiullin AA. Thymoquinone – used in dentistry. International journal of applied and fundamental research. 2016;(12):1660-1662. (In Russ.).
6. Goryainov SV, Khromov AV, Bakureza G, et al. Results of a comparative study of Nigella sativa L. seeds oils composition. Pharmacy & Pharmacology. 2020;8(1):29-39. (In Russ.). DOI:https://doi.org/10.19163/2307-9266-2020-8-1-29-39.
7. Sultana S, Asif HM, Akhtar N, et al. Nigella sativa: Monograph. Journal of Pharmacognosy and Phytochemistry. 2015; 4(4):103–106.
8. Tuna HI, Babadag B, Ozkaraman A, et al. Investigation of the effect of black cumin oil on pain in osteoarthritis geriatric individuals. Complementary Therapies in Clinical Practice. 2018;31:290-294. DOI:https://doi.org/10.1016/j.ctcp.2018.03.013.
9. Khonche A, Huseini HF, Gholamian M, et al. Standardized Nigella sativa seed oil ameliorates hepatic steatosis, aminotransferase and lipid levels in nonalcoholic fatty liver disease: A randomized, double-blind and placebo-controlled clinical trial. Journal of Ethnopharmacology. 2019;234:106-111. DOI:https://doi.org/10.1016/j.jep.2019.01.009.
10. Darand M, Darabi Z, Yari Z, et al. Nigella sativa and inflammatory biomarkers in patients with non-alcoholic fatty liver disease: Results from a randomized, double-blind, placebo-controlled, clinical trial. Complementary Therapies in Medicine. 2019;44:204-209. DOI:https://doi.org/10.1016/j.ctim.2019.04.014.
11. Amin B, Hosseinzadeh H. Black Cumin (Nigella sativa) and Its Active Constituent, Thymoquinone: An Overview on the Analgesic and Anti-inflammatory Effects. Planta Medica. 2016;82:8-16. DOI:https://doi.org/10.1055/s-0035-1557838.
12. Shelestun A, Eliseeva T. Black cumin oil – beneficial and dangerous properties, chemical composition, use in cooking and cosmetology. Journal of Healthy Nutrition and Dietetics. 2022;19. (In Russ.). DOI:https://doi.org/10.59316/oil.2022.19.28.
13. Baibekov RF, Belopukhov SL, Dmitrevskaya II, et al. Comparative characteristics of fatty acids composition in oil lipids from industrial crops seeds. Dostizheniya nauki i tekhniki APK. 2019;33(6):62-65. (In Russ.). DOI:https://doi.org/10.24411/0235-2451-2019-10615.
14. Kaskoos R.A. Fatty acid composition of black cumin oil from iraq. Research Journal of Medicinal Plant. 2011;5(1):85-89. DOI:https://doi.org/10.3923/rjmp.2011.85.89.
15. Todorov Z. Composition and quality of rapeseed oil (Brassica napus oleifera biennis) depending on sowing time and treatment with leaf fertilizers. Scientific Papers. Series A. Agronomy. 2020;LXIII(1):574-579.
16. Issaoui M, Delgado AM. Grading, labeling and standardization of edible oils. Fruit Oils: Chemistry and Functionality. 2019:9-52. DOI:https://doi.org/10.1007/978-3-030-12473-1_2.
17. Ramadan MF, Morsel J. Oxidative stability of black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and niger (Guizotia abyssinica cass.) crude seed oils upon stripping. European Journal of Lipid Science and Technology. 2004;106(1):35-43. DOI:https://doi.org/10.1002/ejlt.200300895.
18. Wang S, Wang J, Dong G, et al. Effect of Different Extraction Methods on Quality Characteristics of Rapeseed and Flaxseed Oils. Journal of Food Quality. 2022;2022. DOI:https://doi.org/10.1155/2022/8296212.
19. Rabadan M, Alvarez-Ortı R, Gomez A, et al. Optimization of pistachio oil extraction regarding processing parameters of screw and hydraulic presses. LWT-Food Science and Technology. 2017;83(15):79–85. DOI:https://doi.org/10.1016/j.lwt.2017.05.006.
20. Luque de Castro MD, Priego-Capote F. Soxhlet extraction: past and present panacea. Journal of Chromatography A. 2010;1217(16):2383-2389. DOI:https://doi.org/10.1016/j.chroma.2009.11.027.
21. Degtereva AYu, Peregonchaya OV, Zvyagin AA. Determination of the acid number of sunflower oil by conductometric titration. Technologies and commodity science of agricultural products. 2017;9(2):149-152. (In Russ.).
22. Lisovaya EV, Viktorova EP. Characteristics of existing methods for determining the acid number of vegetable oils and lecithins. Technologies of food and processing industry of the agro-industrial complex – healthy food products. 2018;1:72-77. (In Russ.).
23. Sharashina AE. Determination of iodine and acid numbers of vegetable oils. Ratio et Natura. 2022;5(1):2. (In Russ.).
24. Burunkova YuE, Uspenskaya MV, Samoylova EO. Vegetable oils: properties, production and storage technologies, oxidative stability. Saint Petersburg: ITMO University; 2020. 82 p. (In Russ.).
25. Kilichov AAU, Muldabekova BJ. Study and analysis of the characteristics of accompanying substances included in vegetable oils. Universum: technical sciences. 2024;120(3):52-54. (In Russ.).
26. Li X, Yang R, Lv C, et al. Effect of chlorophyll on lipid oxidation of rapeseed oil. European Journal of Lipid Science and Technology. 2018;121(4). DOI:https://doi.org/10.1002/ejlt.201800078.
27. Mahoney E, Milewska M, Mironczuk-Chodakowska I, et al. The influence of carotenoid and chlorophyll content on the oxidative processes in the selected vegetable oils. Progress in Health Sciences. 2018;8(2):144-151. DOI:https://doi.org/10.5604/01.3001.0012.8337.
28. Top AG, Muhamad H, Abdullah A, et al. Vitamin E and beta carotene composition in four different vegetable oils. American Journal of Applied Sciences. 2011;8(5):407-412. DOI:https://doi.org/10.3844/ajassp. 2011.407.412.
29. Kulakova SN, Bessonov VV. Possibilities of using vegetable oils of tropical origin. Food industry. 2013;(4):14-17. (In Russ.).
30. Chepura SV, Pluzhnikova NN, Saiganov SA, et al. Mechanisms of Implementation of Alpha-Tocopherol Antioxidant Effects. Advances in modern biology. 2020;140(2):149-165. (In Russ.). DOI: 10.31857/ S0042132420020039.
31. Cardenas E, Ghosh R. Vitamin E: A dark horse at the crossroad of cancer management. Biochemical Pharmacology. 2013;86(7):845-852. DOI:https://doi.org/10.1016/j.bcp.2013.07.018.
32. Shahidi F, De Camargo A. Tocopherols and tocotrienols in common and emerging dietary sources: occurrence, applications, and health benefits. International Journal of Molecular Sciences. 2016;17(10). DOI:https://doi.org/10.3390/ijms17101745.
33. Empey-Kohl J. What to keep in mind when analyzing chlorophyll samples using UV-Vis Spectrometry. ThermoFisher. Scientific. 2025.
34. Rybakova OV, Safonova EF, Slivkin AI. Determination of spectral characteristics of alcohol solutions of vegetable oils and oil extracts by UV spectrophotometry. Vestnik of the Voronezh State University. 2007;2:171-173. (In Russ.).
35. Guidi L, Tattini M, Landi M. How does chloroplast protect chlorophyll against excessive light. InTech eBooks. 2017;3. DOI:https://doi.org/10.5772/67887.
36. Protopopov AV, Shleina AN, Kuris YuE, et al. Thermal modification of vegetable oils in the presence of sulfur. Polzunovsky Vestnik. 2017;4:21-25. (In Russ.).
37. Kasote DM, Badhe YS, Hegde MV. Effect of mechanical press oil extraction processing on quality of linseed oil. Industrial Crops and Products. 2012;42:10-13. DOI:https://doi.org/10.1016/j.indcrop.2012.05.015.
38. Zeng J, Xiao T, Ni X, et al. The comparative analysis of different oil extraction methods based on the quality of flaxseed oil. Journal of Food Composition and Analysis. 2022;107. DOI:https://doi.org/10.1016/j.jfca. 2021.104373.
39. Muangrat R, Kaikonjanat A. Comparative evaluation of hemp seed oil yield and physicochemical properties using supercritical CO2, accelerated hexane, and screw press extraction techniques. Journal of Agriculture and Food Research. 2024;19. DOI:https://doi.org/10.1016/j.jafr.2024.101618.
40. Tatochenko IM, Tatochenko AL, Chernegov NYu. On the issue of choosing technological equipment for processing food hemp seeds – the economic aspect of the problem. Modern Science. 2019;(8-2):96-102. (In Russ.).
41. Belopukhov SL, Baibekov RF, Zharkikh OA. Chemical composition of oil from hemp seeds of the Surskaya variety. Science Bulletin. 2019;18(9):57-59. (In Russ.).
42. Jiang L, Wu W, Wu S, et al. Effect of different pretreatment techniques on quality characteristics, chemical composition, antioxidant capacity and flavor of cold-pressed rapeseed oil. LWT. 2024;201. DOI:https://doi.org/10.1016/j.lwt.2024.116157.
43. Agah MS, Azadmard-Damirchi S, Bodbodak S. Quality of oil extracted by cold press from Nigella sativa seeds conditioned and pre‐treated by microwave. Food Science & Nutrition. 2024;12(5):3529-3537. DOI:https://doi.org/10.1002/fsn3.4021.
44. Abedinzadeh S, Torbati M, Azadmard-Damirchi S, et al. Changes in the quality of oil extracted by hot pressing from black cumin (Nigella sativa) seeds and by solvent from the obtained cake during refining. Food science & nutrition. 2024;12(5):3563-3573. DOI:https://doi.org/10.1002/fsn3.4025.
45. Sicaire AG, Vian MA, Fine F, et al. A review of methods used for seed oil extraction. Indian Journal of Experimental Biology. 2016;54(9):569-580. DOI:https://doi.org/10.21275/1121804.
46. Hasanov JH, Mirzaxmedov SD, Sultonova EM. Effect of moisture content on the quality and quantity of screw-pressed flax seed oil. Food Processing: Techniques and Technology. 2023;53(2):309-315.
47. Tarasevičienė Ž, Laukagalis V, Paulauskienė A, et al. Quality changes of cold-pressed Black Cumin (Nigella sativa L.), Safflower (Carthamus tinctorius L.), and Milk Thistle (Silybum marianum L.) seed oils during storage. Plants. 2023;12(6). DOI:https://doi.org/10.3390/plants12061351.
48. The State Pharmacopoeia of the Russian Federation. 14 ed. Moscow; 2018. Vol. 2. 3262 p.
49. Alander J, Lidefelt JO. Handbook: Vegetable oils and fats (2nd ed.). Karlshamn: AarhusKarlshamn; 2007. 253 p.
50. Marudova M, Viraneva A, Antova G, et al. Physico-Chemical characterization of Sesame/Rapeseed oil mixtures. Applied Sciences. 2025;15(2). DOI:https://doi.org/10.3390/app15020704.
51. Farhan N, Salih N, Salimon J. Physiochemical properties of Saudi Nigella sativa L. (‘Black cumin’) seed oil. OCL. 2021;28(11). DOI:https://doi.org/10.1051/ocl/2020075.
52. Kuregyan AG. Spectrophotometry in the analysis of carotenoids. Fundamental research. 2016;23(2):5166-5172. (In Russ.).
53. Peregonchaya OV, Korolkova NV. Spectrophotometric study of the composition of the pigment complex of rapeseed oil. Technologies and commodity science of agricultural products. 2020;14(1):155-158. (In Russ.).



