employee from 01.01.2022 to 01.01.2025
Vyatka State University (Institute of Chemistry and Ecology, associate professor)
employee from 01.01.2017 to 01.01.2025
employee from 01.01.1988 to 01.01.2025
VAK Russia 4.1.1
VAK Russia 4.1.2
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
VAK Russia 4.3.3
VAK Russia 4.3.5
UDC 57.04
UDC 633.162
UDC 57.084.1
CSCSTI 34.00
CSCSTI 62.00
The aim of the study is to comparatively evaluate the response of regenerated and original barley genotypes to soil stressors in terms of changes in morphometric and biochemical parameters of leaves. The work was carried out in 2024 at the Federal Scientific Center of the North-East (Kirov, Kirov Region, Russia). The object of the study was spring barley (Hordeum vulgare L.) variety ‘Boyarin’ and its second-generation regenerated lines. Sod-podzolic loamy soil was used for the experiment. Four soil backgrounds were simulated: control, with excess cadmium, acidic, and drought. Plants were grown under natural conditions. Leaves for the study were selected at the heading stage. Leaf length positively correlated with productivity (r = 0.49) against an acidic background; negatively – against a soil background containing cadmium and under drought (r = –0.48 and r = –0.75). Changes in leaf length and area were closely related to productivity in the original variety (r = 0.77...0.75) and the RA-PEG regenerant (r = 0.99...0.82). Regardless of soil conditions, the original variety was characterized by 45–73 % lower chlorophyll a content than the regenerants; 67–110 % lower chlorophyll b content; 40–80 % lower carotenoids; and 12–15 % lower total phenolic compound (PC) content. Compared with the original form, the regenerants had a higher antioxidant potential, diagnosed by the content of photosynthetic pigments and PC in the leaves. The greatest heterogeneity of genotypes in the total PC content in the leaves (CV = 10.7 %) was observed under drought. The content of chlorophyll a and carotenoids in the leaves did not differ statistically on different soil backgrounds; chlorophyll b increased by 50 % compared to the control under cadmium soil contamination. A conclusion was made about the effectiveness of the applied in vitro selection methods in obtaining regenerative genotypes capable of maintaining productivity under the influence of soil stressors.
barley, length, area, chlorophyll, phenolic compounds, subflag leaf, acidity, drought, cadmium
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