Ryazan, Orel, Russian Federation
VAK Russia 4.1.1
VAK Russia 4.1.2
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
VAK Russia 4.3.3
VAK Russia 4.3.5
UDC 636.1
CSCSTI 68.00
Russian Classification of Professions by Education 36.00.00
Russian Library and Bibliographic Classification 4
Russian Trade and Bibliographic Classification 5622
BISAC TEC003020 Agriculture / Animal Husbandry
The aim of this study was to investigate the genetic structure of the Novo-Altai breed at the Extension and Agouti loci, which control skin and hair pigmentation. The Novo-Altai horse is a productive breed kept in herds. The adaptive qualities of herd horses are influenced by numerous genetic factors, including genes regulating pigmentation. Hair samples with hair follicles were collected from 80 Novo-Altai horses bred on breeding farms in the Altai Republic and Altai Region. The samples were genotyped at the Extension and Agouti loci using PCR-RFLP technology. Statistical analysis of the results was carried out to determine the frequency of alleles, the observed heterozygosity of the studied loci, and the frequency of genotypes in the population. The frequency of the dominant allele "E" and the recessive allele "e" at the Extension locus was 0.3875 and 0.6125, respectively. At the Agouti locus, the frequency of the dominant allele "A" was 0.4875, and the recessive allele "a" was 0.5125. The observed heterozygosity values were 0.475 at the Extension locus and 0.500 at the Agouti locus. Taking into account the results of testing at 2 loci, 9 different genotype variants were identified in the studied group of animals, the frequency of which varied from 0.2750 (genotype "E/e, A/a") to 0.0375 (genotype "E/E, a/a"), indicating a high level of polymorphism in the population. The most popular basic coat color in the Novo-Altai breed was bay. The total frequency of the four genotypes associated with the bay coat colour in the population was 0.4750.
domestic horses, Novoaltai horses, coat colours, Extension, Agouti
1. Kalashnikov RV, Kalashnikov VV. Herd horse breeding in Russia. Konevodstvo i konnyi sport. 2011;5:6-9 (In Russ.).
2. Bailey EF, Brooks SA. Horse genetics, 3rd edition. CABI, 2020.
3. Liu X, Peng Y, Zhang X, et al. Coloration in Equine: Overview of Candidate Genes Associated with Coat Color Phenotypes. Animals. 2024;14(12):1802. DOI:https://doi.org/10.3390/ani14121802. EDN: https://elibrary.ru/IPYHCV.
4. Ludwig A, Pruvost M, Reissmann M, et al. Coat color variation at the beginning of horse domestication. Science. 2009;324(5926):485. DOI:https://doi.org/10.1126/science.1172750. EDN: https://elibrary.ru/MNGRQZ.
5. Reissmann M. Die Farben der Pferde. Cadmos, 2009.
6. Librado P, Gamba C, Gaunitz C, et al. Ancient genomic changes associated with domestication of the horse. Science. 2017;356(6336):442-445. DOI:https://doi.org/10.1126/science.aam5298. EDN: https://elibrary.ru/XNKBSA.
7. Linderholm A, Larson G. The role of humans in facilitating and sustaining coat colour variation in domestic animals. Seminars in Cell & Developmental Biology. 2013;24(6-7):587-593. DOI:https://doi.org/10.1016/j.semcdb.2013.03.015. EDN: https://elibrary.ru/RHFYAB.
8. Rieder S. Molecular tests for coat colours in horses. Journal of Animal Breeding and Genetics. 2009;126(6):415-424. DOI:https://doi.org/10.1111/j.1439-0388.2009.00832.x. EDN: https://elibrary.ru/MYOLBH.
9. Chowdhary BP. Equine genomics. Wiley-Blackwell; 2013.
10. McFadden A, Vierra M, Martin K, et al. Spotting the Pattern: A review on white coat color in the domestic horse. Animals. 2024;14(3):451. DOI:https://doi.org/10.3390/ani14030451. EDN: https://elibrary.ru/KFQRAP.
11. Sponenberg DP, Bellone R. Equine color genetics. Willey-Blackwell; 2017.
12. Marklund L, Moller MJ, Sandberg K, et al. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses. Mammalian Genome. 1996;7:895-899. DOI:https://doi.org/10.1007/s003359900264. EDN: https://elibrary.ru/GIOWPE.
13. Rieder S, Taourit S, Mariat D, et al. Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus). Mammalian Genome. 2001;12:450-455. DOI:https://doi.org/10.1007/s003350020017. EDN: https://elibrary.ru/ATDQSD.
14. Kalinkova LV, Kuptsova NA. Genetic characterization of the Russian population of German classic ponies by STR loci and genes affecting coat colour. Agricultural Biology. 2024;59(6):1108-1117 (In Russ.). DOI:https://doi.org/10.15389/agrobiology.2024.6.1108rus. EDN: https://elibrary.ru/LEKVOG.
15. Kalinkova LV, Zaitsev AM, Ivanov RV. Genetic structure of the local Yakutian horse population for genes MC1R, ASIP, DMRT3, and MSTN. Agricultural Biology. 2022;57(2):272-282 (In Russ.). DOI:https://doi.org/10.15389/agrobiology.2022.2.272rus. EDN: https://elibrary.ru/DSAGID.
16. Avila F, Hughes SS, Magdesian KG, et al. Breed distribution and allele frequencies of base coat color, dilution, and white patterning variants across 28 horse breeds. Genes. 2022;13(9):1641. DOI:https://doi.org/10.3390/genes13091641. EDN: https://elibrary.ru/TYVXSK.
17. Kalinkova LV. Study of polymorphism of the ASIP and MC1R genes in Arabian horses. Genetics and breeding of animals. 2020;2:50-53 (In Russ.). DOI:https://doi.org/10.31043/2410-2733-2020-2-50-53. EDN: https://elibrary.ru/PCRHUJ.
