Leuzea safflower, or maral root (Rhaponticum carthamoides), is a perennial herbaceous plant, listed in the Red book and protected by law. In its composi-tion there are pharmacologically important compo-nents. For the tinctures production rhizome is used, as a result the plant dies. In the study the possibility of using callus tissue of Leuzea safflower as alterna-tive raw material for the production of tinctures, drinks, etc. is considered. The aim of the study was to establish the dependency of the yield of extractives from callus tissue and the source of the plant Leuzea safflower from various process parameters. The re-search problems were the definition of exit of extrac-tive substances from the concentration of the solvent, the hydraulic module, the duration of extraction, par-ticle size distribution. The results of studies on the definition of the yield of extractives from callus tissue and the source of the plant depending on the different technological parameters (the duration of infusion, particle size, the concentration of the solvent, liquid module) were given. It was established that the greatest exit of extractive substances from callus tis-sue and initial plant was under the following extrac-tion conditions: the concentration of the extragent (ethyl alcohol) was 40 %, the size of callus tissue particles was from 2.0 to 2.5 mm, the hydraulic mod-ule was 10 and the extraction duration was 2.5 h.