In the article the problems of nitrates content in tubers of vegetable cultures and potatoes in agroecosystem of a garden-kitchen garden of a forest-steppe zone of Krasnoyarsk region are considered. Nitrates are an integral part of all land and water ecosystems as the process of nitrification conducting to formation of the oxidized inorganic form of nitrogen is the basic mechanism having global character. Nitrates are extremely unevenly distributed organs and tissues of a plant. The objects of research were tubers of vegetable cultures (carrots, beet) and potatoes, grown up in agroecosystems of a garden-kitchen garden, in the neighborhood of the villages of Balachta, Novoselovo, Beryozovka, Yemelyanovo and the cities of Bogotol, Sosnovoborsk of a forest-steppe zone of Krasnoyarsk region. Researches were conducted in September, 2012-2014. The objective of the research was to define the content of nitrates of tubers of vegetable cultures (carrots, beet) and potatoes in agroecosystems of a garden-kitchen garden of a forest-steppe zone of Krasnoyarsk region. The main method of research was the environmental monitoring allowing analyzing natural ecosystems and agroecosystems on biotic indicators for their environmental control. During researches it is revealed that potatoes, carrots and beet have different contents of nitrates in various regions of studies. The greatest content of nitrates in all studied cultures is observed in Sosnovoborsk, Bogotol and the village of Beryozovka; an average was in the village of Yemelyanovo, and the smallest was in the villages of Balakhta and Novoselovo. The use of high-yielding grades which are capable of intensive accumulation of nitrates and increase of the brought doses of nitrogen-containing fertilizers, both inorganic, and organic can serve one of the explanations of it.